Gadvasu

Faculty Detail

Academic Credentials

• B.V.Sc. & A.H. (MAFSU, Nagpur)
  
• M.V.Sc. (Animal Biotechnology, ICAR-IVRI, Izatnagar)
  
• Ph.D. (Animal Biotechnology, ICAR-IVRI, Izatnagar)

Teaching and Other Appointments

Specialization: Animal Biotechnology.

• Teaching courses related to Molecular Diagnostics, Gene Cloning & Expression, Functional Genomics & Proteomics. My teaching interests include Genetic Engineering, Molecular Cell Biology, Cell culture and Immunology.

Area of Research Interests

• Molecular Virology (Diagnostic & Vaccinology), Gene Therapy and Cell line development. Currently, I am working in the area of next generation vaccine and diagnostic development for viral diseases. I am also involved in development of stable cell line (Lentivirus transduced) for specific purpose.
  

Ongoing Projects

SN	Name of Project/ Scheme	Funding Agency	Period (from – to)	Total Funding	Role of faculty
1.	Development of user-friendly diagnostic kit for Marek’s disease.	UGC, India	2014-2018	Rs. 6.91 Lakhs	PI
2.	Development of sub-viral particles of Infectious Bursal Disease virus as a potential vaccine and diagnostic candidate.	DBT, India	2014-2018	Rs. 26.35 Lakhs	PI
3.	Development of subviral particle of Japanese encephalitis virus (JEV) as a candidate vaccine against JEV.	DAE, India	2014-2018	Rs. 16.4 Lakhs	PI
4.	Development of virus-like particle (VLP) of Japanese encephalitis virus as a potential vaccine candidate	DBT, India	2013-2017	38.276 Lakh	PI

Research Honour’s /Awards

• Best poster award, XXIII conference, IAVMI.
• Best poster award, XVIII conference, IAAVR.
• Young Scientist Award, PGIMR-Young Scientist Award, VIROCON.
• Young Scientist Award, XIX conference, ISVIB.
• Best Biotechnologist Award, Vet Club, Ludhiana.
• Best poster award, XXXIV conference, IAVMI. 
  

No. of Publications

Research: 27	Extension: Nil	Books: Nil	Manuals: Nil

Publications

• Dubey S, Singh A, Kumar BTN, Singh NK, Tyagi A. (2021) Isolation and Characterization of Bacteriophages from Inland Saline Aquaculture Environments to Control Vibrio parahaemolyticus Contamination in Shrimp. Indian J Microbiol. 61(2):212-217.

• Gupta PK, Sonwane AA, Singh NK, Meshram CD, Dahiya SS, Pawar SS, Gupta SP, Chaturvedi VK, Saini M. (2012) Intracerebral delivery of small interfering RNAs (siRNAs) using adenoviral vector protects mice against lethal peripheral rabies challenge. Virus Res. 163(1):11-8.
  
• Kaur B, Naveen Kumar BT, Tyagi A, Admane Holeyappa S, Singh NK. (2021) Identification of novel vaccine candidates in the whole-cell Aeromonas hydrophila biofilm vaccine through reverse vaccinology approach. Fish Shellfish Immunol. 29;114:132-141.
• Jyoti, Singh NK, Singh H, Singh NK, Rath SS. (2021)  Genotyping amitraz resistance profiles in Rhipicephalus microplus Canestrini (Acari: Ixodidae) ticks from Punjab, India.Ticks Tick Borne Dis. 12(1):101578.
• Jyoti, Singh NK, Singh H, Singh NK, Rath SS.(2016) Multiple mutations in the acetylcholinesterase 3 gene associated with organophosphate resistance in Rhipicephalus (Boophilus) microplus ticks from Punjab, India.Vet Parasitol. 30;216:108-17.
• Kaur N, Singh H, Sharma P, Singh NK, Kashyap N, Singh NK. (2020) Development and application of multiplex PCR assay for the simultaneous detection of Babesia vogeli, Ehrlichia canis and Hepatozoon canis in dogs.  Acta Trop. doi: 10.1016/j.actatropica.2020.105713.
• Leishangthem GD, Singh NK, Singh ND, Filia G, Singh A. (2018) Cell free mitochondrial DNA in serum and milk associated with bovine mastitis: a pilot study. Vet Res Commun. 42(4):275-282.
• Meshram CD, Singh NK, Sonwane AA, Pawar SS, Mishra BP, Chaturvedi VK, Saini M, Singh RP, Gupta PK. (2013) Evaluation of single and dual siRNAs targeting rabies virus glycoprotein and nucleoprotein genes for inhibition of virus multiplication in vitro.Arch Virol. 158(11):2323-32.
• Pawar SS, Meshram CD, Singh NK, Saini M, Mishra BP, Gupta PK. (2017) EvaGreen-based Multiplex Real-time PCR Assay for Rapid Differentiation of Wild-Type and Glycoprotein E-Deleted Bovine Herpesvirus-1 Strains. Anim Biotechnol. 28(4):248-252.
• Pawar SS, Meshram CD, Singh NK, Saini M, Mishra BP, Gupta PK. (2015) Loop-Mediated Isothermal Amplification for Rapid Detection and Differentiation of Wild-Type Bovine Herpesvirus-1 and Glycoprotein E-Deleted Marker Vaccine Strain. Anim Biotechnol. 26(4):268-72.
• Rai S, Tyagi A, Kalia A, Kumar BTN, Garg P, Singh NK. (2020) Characterization and genome sequencing of three Aeromonas hydrophila-specific phages, CF8, PS1, and PS2. Arch Virol. 165(7):1675-1678.
• Rai S, Tyagi A, Kalia A, Kumar BTN, Garg P, Singh NK. (2020) Correction to: Characterization and genome sequencing of three Aeromonas hydrophila-specific phages, CF8, PS1, and PS2. Arch Virol. 165(8):1933-1934.
• Saugandhika S, Sharma V, Malik H, Saini S, Bag S, Kumar S, Singh NK, Mohanty AK, Malakar D. (2015) Expression and purification of buffalo interferon-tau and efficacy of recombinant buffalo interferon-tau for in vitro embryo development. Cytokine. 75(1):186-96.
• Singh MD, Singh H, Singh NK, Singh NK, Kashyap N, Sood NK, Rath SS. (2019) Development of loop-mediated isothermal amplification (LAMP) assay for detection of Hepatozoon canis infection in dogs. Ticks Tick Borne Dis. 10(2):371-376.
• Singh MD, Singh H, Singh NK, Singh NK, Sood NK, Rath SS. (2020) Evaluation of a Loop-Mediated Isothermal Amplification Technique for the Rapid Visual Detection of Hepatozoon canis Infection. Acta Parasitol. 65(1):151-155.
• Singh NK, Tyagi A, Kaur R, Verma R, Gupta PK. (2016) Characterization of codon usage pattern and influencing factors in Japanese encephalitis virus.Virus Res. 2016 Aug 2;221:58-65.
• Singh NK, Tyagi A, Kaur R, Verma R, Gupta PK. (2016) Characterization of codon usage pattern and influencing factors in Japanese encephalitis virus.Virus Res. 221:58-65.
• Singh NK, Tyagi A. (2017) A detailed analysis of codon usage patterns and influencing factors in Zika virus.Arch Virol. 162(7):1963-1973.
• Tyagi A, Kumar BTN, Singh NK. (2017) Genome dynamics and evolution of codon usage patterns in shrimp viruses. Arch Virol. 162(10):3137-3142.
• Tyagi A, Singh B, Billekallu Thammegowda NK, Singh NK. (2019) Shotgun metagenomics offers novel insights into taxonomic compositions, metabolic pathways and antibiotic resistance genes in fish gut microbiome. Arch Microbiol. 201(3):295-303.
• Tyagi A, Singh NK, Gurtler V, Karunasagar I. (2016) Bioinformatics analysis of codon usage patterns and influencing factors in Penaeus monodon nudivirus. Arch Virol. 161(2):459-64.
• Padmaja M, Singh H, Panwar H, Singh NK, Singh NK. (2022) Development and validation of multiplex SYBR Green real-time PCR assays for detection and molecular surveillance of four tick-borne canine haemoparasites. Ticks and Tick-borne Diseases 13, 101937.
• Jamwal S, Singh NK. (2021) Expression and Purification of Exposed Outer Domain of Pseudomonas aeruginosa Opr86 protein. Journal of Animal Research 11, 571-577.